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SOLVED: Using the equation for calculating the melting temperature (Tm) for primers, calculate the Tm's for each primer in degrees Celsius. Apart from the melting temperature of the primers, describe two other
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Sequences and melting temperature (Tm) of PCR primers used in this study. | Download Table
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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Primers and Tm for genes analysed with Real Time PCR. | Download Table
Should we optimise annealing temperature for ready PCR mastermix? | ResearchGate
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Primer sequences and annealing temperature (Tm) for amplification and... | Download Table
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